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Fructose-1,6-bisphosphatase (FBPase, EC 3.1.3.11) is a key enzyme in the glycolytic pathway. It catalyzes the conversion of fructose-1,6-bisphosphate to fructose 6-phosphate and inorganic orthophosphate. FBPase is encoded by a single gene in mammals and, like other metabolic enzymes involved in energy metabolism, it is expressed in a variety of tissues and cells. Type IIa isozyme of FBPase (FBPAIIa) has unique properties among isozymes, particularly among the liver isoforms. It has a weaker affinity for fructose-1,6-bisphosphate (Km = 7-8 mM) and a lower specific activity in liver mitochondria than the liver type IIa isozyme, which has Km values of 8-13 mM and specific activity of 200-300 nmol/min/mg protein. The purified FBPAIIa has optimum pH, Km for fructose-1,6-bisphosphate and Mg2+ between 7.4 and 8.0, and optimum temperature at 30-35 degrees C. It is present in brain with high concentration in the olfactory ensheathing cells (OECs). The purified enzyme binds to pyridoxal-5′-phosphate (PLP) specifically, in a highly cooperative manner. Treatment of OECs with neural growth factor (NGF) decreases the specific FBPAIIa activity and causes a significant shift of type IIa to type I isozyme in mitochondria. The decrease in the enzyme activity correlates with a significant shift of FBPase activity from type IIa to I isozyme. Thus, neural regulation of FBPase activity in brain may occur at a post-translational level. Moreover, the type IIa isozyme appears to be the predominant form in cultured OEC

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