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An inflammatory response in the airways is a key feature of acute and chronic lung diseases associated with airway epithelial damage. However, the response of epithelial cells exposed to inhaled gases is less clear. H292 cells were exposed to increasing concentrations of CO and O(2) in air for 24 h. Apoptotic cells were measured with annexin V and propidium iodide staining and endoplasmic reticulum stress was assessed by using the anti-Bip and calpain 12 antibodies. The sensitivity of the cells for apoptosis in response to CO and O(2) exposure was similar with 1% CO or 6% O(2), and at a higher concentration (4%) of both gases there was a reduced sensitivity. Apoptosis was inhibited by the caspase inhibitor zVAD but, unlike staurosporine, had no effect on CO-induced apoptosis at any concentration tested. Moreover, the calpain inhibitor MDL-28170 had no effect on apoptosis in response to CO or O(2). CO and O(2) exposure increased the expression of endoplasmic reticulum chaperone BiP (GRP78) in a concentration-dependent manner. However, O(2) had a greater effect at any concentration, and at the highest concentration tested there was an over

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