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A direct correlation between cellular proliferation and IFN-alpha-mediated growth suppression is established for a number of tumors. To test whether a similar relationship exists for human melanoma, the authors examined the effects of IFN-alpha on the growth of a variety of human melanoma cell lines in vitro and in a xenograft model in vivo. Fourteen cell lines were assayed for IFN-alpha-mediated growth inhibition. Cells were exposed to recombinant human IFN-alpha for a period of time ranging from 24 to 96 hr. Proliferation of the tumors was measured by 3H-thymidine incorporation, and tumor volumes and IFN-alpha binding was measured by immunofluorescence. To examine IFN’s antitumor effects in vivo, human melanoma cells were implanted into nude mice and then injected with increasing doses of IFN-alpha. Melanoma cell growth was assessed by tumor weight and 3H-thymidine incorporation. Tumor volume was assessed by caliper measurements. Seventy-five percent of melanoma cell lines were growth inhibited by IFN-alpha treatment. The extent of IFN-alpha-mediated growth inhibition correlated well with tumor cell proliferation in vitro. In vivo IFN-alpha had a dose-dependent effect on human melanoma growth. Antitumor activity was increased with prolonged exposure to IFN-alpha. Human melanoma appears to be an ideal tumor for the study of IFN-alpha-mediated growth inhibition, as IFN’s actions were consistently seen and were very reproducible.Assisted Living in Piedmont, NV

Assisted Living Communities in Piedmont, Nevada

Piedmont has a population of 3,610 and is located in Washoe County.

There are 24 assisted living facilities in Piedmont, with a total of 834 assisted living units. The typical monthly cost of assisted living in Piedmont is around $3,155. This is higher than the median cost of $2,744 for all Nevada counties and lower than the median cost for all counties in the country.

Piedmont is
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