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Using a combination of immunocytochemistry and molecular biological techniques, we have characterized the distribution of the nine rat nicotinic acetylcholine receptor (nAChR) subunits in the neurons of the dorsal root ganglion (DRG) of the adult rat. Double-labeling studies show that the neuron-specific enolase (NSE), a highly abundant gamma-type nicotinic acetylcholine receptor subunit, and the vesicular gamma-aminobutyric acid transporter (GABA-T) are co-localized in most of the DRG neurons. The distribution of subunits of the beta2 subunit-containing nAChR at the ultrastructural level indicates that these receptors are preferentially located on the postsynaptic membrane of the neurons of the DRG. Immunocytochemical studies of the distribution of beta2 subunits in the DRG show that these subunits have a co-localization pattern similar to the NSE, consistent with a functional co-localization of nAChRs and GABA-T in the DRG neurons. On the other hand, there is no co-localization of the muscle nAChR alpha1 subunit with any of the above markers. The vesicular acetylcholine transporter (VAChT), a marker for cholinergic neurons, as well as choline acetyltransferase (ChAT) and cholinesterase, a marker for cholinergic neurons, are found in some DRG neurons; however, their distributions are clearly distinct from that of any of the nAChR subunits.The City of Roseville has approved a plan to fix a flooded segment of Alder Creek, making way for wider sidewalks and a bike and pedestrian path along the creek.

About 65,000 cubic yards of soil needs to be removed during construction of the Alder Creek Project, the city said Thursday. That’s enough to fill “a hole, two or three football fields,” said Tom Bompensiero, director of public works.

The project is expected to begin in early 2020. Bompensiero said the
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