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Feruloylation of xylan polymer chain is catalyzed by a cell wall-bound enzyme called feruloyl esterase (FAE). Biosynthesis of feruloylated or sinapoylated monolignols starts with the conversion of coniferyl alcohol (CA) or syringyl alcohol (SA) into the aldehyde form. Oxidative polymerization of aldehydes as well as autoxidation lead to the formation of ferulic and sinapic acids, respectively. The physiological regulation of this process has been intensively investigated. CA and SA are preferentially supplied to FAE from this polymer, whereas the supply of the respective aldehydes from free CA and SA forms is more limited. CA biosynthesis thus appears favored in etiolated soybean seedlings whereas SA cannot replace CA in seedlings exposed to blue light. Also, redox status of seedlings, e.g., caused by auxin exposure or the removal of the hydrogen donor ascorbic acid, differently affect the biosynthesis of CA and SA. Auxin induces the synthesis of sinapic acid, whereas ascorbic acid is an inhibitor. Intermolecular cross-linking between phenolic acids increases under conditions of high oxidative stress, e.g., caused by exposure to alkaline pH or H2O2. They can subsequently be used as substrates for FAE.Q:

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